PRX5 Role in Colorectal Cancer Stemness and Tumorigenicity

A biomedical research dataset investigating peroxiredoxin 5 (PRX5) as a regulator of cancer stem cells in colorectal cancer. The dataset includes results from in vitro assays and in vivo xenograft studies in BALB/c-nu mice, authored by Sung Woo Lee and last updated in May 2026. The data likely contains measurements of gene expression, reactive oxygen species, and tumor growth metrics.

Use Cases

• Analyze the relationship between PRX5 expression and pluripotency markers (OCT4, SOX2, NANOG, CD133) based on qPCR and western blot data.
• Investigate the effect of PRX5 knockdown on sphere-forming frequency based on extreme limiting dilution analysis (ELDA).
• Model tumor growth dynamics based on in vivo xenograft volume and weight measurements.
• Study the link between PRX5-mediated redox environment and STAT3 activation based on p-STAT3 immunoblot data.

Strengths

• Includes in vivo validation with specific tumor growth metrics (2.5-fold greater volume, 1.82-fold higher weight).
• Reports high-efficiency gene knockdown (>95%) for PRX5.
• Data is openly licensed under CC-BY-4.0.

Limitations

• Column-level documentation is absent; field semantics must be inferred after download.
• Row count is unknown, which may limit suitability assessment.
• The dataset is small in size (938.4 KB), suggesting limited scope.

Provenance

Source

figshare

Collection Method

Experimental data from HCT116 cell line models, serum-free spheroid culture, siRNA/shRNA gene disruption, and mouse xenografts.

Freshness

Last updated 2026-05-16 13:20:03; freshness should be verified.