Pancreatic Zymogen Granule Purification Protocol Using Percoll Gradients

A procedure for purifying pancreatic zymogen granules in large quantity based on Percoll gradient centrifugation. The method is described by Xuequn Chen and John A. Williams from the University of Michigan. Percoll, a non-toxic colloidal silica with a density of 1.130 g/ml, is used to separate organelles with densities greater than 1.060 g/ml.

Use Cases

• Isolating highly purified zymogen granules based on the described Percoll gradient method.
• Separating zymogen granule membrane and content following the purification protocol.
• Adapting the procedure for purifying smaller amounts of granules from isolated pancreatic acini as per the described modification.

Strengths

• The protocol is based on a widely used and non-toxic separation medium (Percoll).
• The description cites multiple prior laboratory implementations (references 1-4, 6-10, 12-16).
• Includes a modification for working with smaller sample quantities from isolated acini.

Limitations

• Row count and dataset scale are unknown, which may limit suitability assessment.
• Column-level documentation is absent; field semantics must be inferred after download.
• Last update date is unknown; freshness unverified.

Provenance

Source

University of Michigan

Collection Method

Percoll gradient centrifugation protocol for organelle separation.