qPCR in Human Eosinophils After Glucocorticoid Exposure

Five human subjects provided peripheral blood for eosinophil isolation. Purified cells were incubated for 4 hours, then exposed to dexamethasone or vehicle for 30, 60, or 120 minutes before RNA extraction and qPCR analysis for 11 target genes. The dataset presents mean Ct values for each subject, condition, and time point, calculated from two technical replicates per sample.

Use Cases

• Modeling time-dependent gene expression changes based on dexamethasone exposure duration
• Comparing glucocorticoid response across 11 target genes including CXCR4, CCR1, and BCL2L11
• Analyzing inter-subject variability in eosinophil gene expression under controlled conditions

Strengths

• Data includes two technical replicates per measurement, which may improve reliability
• Experimental design includes multiple time points (30, 60, 120 minutes) for tracking response dynamics
• Analysis covers 11 specific human genes relevant to immune function and apoptosis

Limitations

• Row count is unknown, which may limit suitability assessment
• Column-level documentation is absent; field semantics must be inferred after download
• Last update date is unknown; freshness unverified

Provenance

Source

Luis M. Franco via paperswithcode

Collection Method

Eosinophils were isolated from human peripheral blood, treated with dexamethasone or vehicle, followed by RNA purification and qPCR.