Na,K-ATPase Expression: Super-Resolution PALM/STORM Imaging and Density Data

Super-resolution PALM/STORM imaging data quantifying Na,K-ATPase α1 isoform expression, authored by Kristoffer Bernhem and released in 2023. The data tracks protein density and localization changes in the plasma membrane over a 41-hour transfection period.

Use Cases

• Quantifying plasma membrane protein density using STORM/PALM localization data
• Comparing endogenous vs. exogenous protein contributions via N-terminal hemagglutinin epitope tags
• Analyzing the spatial distribution and cluster size of Na,K-ATPase α1 isoform over time

Strengths

• Uses super-resolution PALM/STORM imaging for sub-diffraction spatial analysis
• Includes specific quantification of a 63% increase in membrane density after 41h
• Features dual-labeling with mMaple3 and hemagglutinin epitopes

Limitations

• Small scope focused exclusively on the Na,K-ATPase α1 isoform
• Missing metadata regarding the total number of images or records
• Temporal resolution is restricted to specific transfection time points like 41 hours

Provenance

Source

Kristoffer Bernhem via Dryad

Collection Method

Super-resolution PALM/STORM microscopy of transiently transfected cells

Freshness

Last updated 2023-08-18.