Histone Modification Quantification in Human TK6 Cells via LC-MS/MS

A new liquid chromatography-coupled tandem mass spectrometry method quantified N⁶-methyl-, -acetyl-, and -formyl-lysine modifications on histone proteins from human TK6 cells. The study reports N⁶-formyllysine levels of 1–4 modifications per 10⁴ lysines, contrasting with acetylation and methylation levels ranging from 1.5 to 1400 per 10⁴ lysines. The research was conducted by Bahar Edrissi of the Massachusetts Institute of Technology.

Use Cases

• Modeling the relationship between formaldehyde exposure and N⁶-formyllysine levels based on the dose-dependent increase described.
• Analyzing the distribution of lysine modifications across histone protein classes based on the reported quantification per 10⁴ lysines.
• Investigating the enzymatic activity of histone deacetylases on N⁶-formyllysine substrates based on the reported minimal SIRT1 activity.
• Comparing the abundance of pathological N⁶-formyllysine to physiological acetylation and methylation modifications.

Strengths

• Quantifies three specific lysine modifications (N⁶-methyl-, -acetyl-, and -formyl-lysine) with precise reported levels per 10⁴ lysines.
• Includes data on modification levels across different classes of histone proteins from a defined human cell line (TK6).
• Presents results from isotope labeling studies using [¹³C,²H₂]-formaldehyde to distinguish endogenous and exogenous adduct sources.

Limitations

• Row count and dataset scale are unknown, which may limit suitability assessment for large-scale ML tasks.
• Column-level documentation is absent; field semantics must be inferred after download.
• Last update date is unknown; freshness unverified.

Provenance

Source

Massachusetts Institute of Technology

Collection Method

Liquid chromatography-coupled tandem mass spectrometry (LC-MS/MS) on histone proteins from human TK6 cells.