Human Kidney Tissue N-Glycosylation Patterns in Minor Glomerular Abnormalities

A 2026 study by Yingying Ling compares intact N-glycopeptide profiles from 27 trace renal biopsy samples of patients with minor glomerular abnormalities against 24 distant non-neoplastic tissue samples. Integrated high-resolution mass spectrometry quantified 672 upregulated and 573 downregulated glycopeptides, revealing distinct glycosylation patterns. The dataset provides site-specific N-glycosylation analysis for proteins including IgG subclasses and complement-related markers.

Use Cases

• Identify differentially expressed intact N-glycopeptides (IGPs) with fold change >1.5 or <0.67 and p-value <0.05 between MGA and control tissues.
• Analyze site-specific N-glycosylation patterns for 24 glycoproteins associated with the PI3K-Akt signaling pathway to understand pathway dysregulation.
• Compare glycosylation signatures of IgG subclasses and complement-related markers to distinguish minor glomerular abnormalities from healthy tissue.
• Validate pressure cycling technology-based glycoproteomics workflows for trace-tissue analysis using quantified IGP abundances.

Strengths

• Quantifies 1,245 significantly altered intact N-glycopeptides (672 upregulated, 573 downregulated).
• Compares 51 total human tissue samples (27 MGA patient biopsies, 24 control tissues).
• Provides site-specific N-glycosylation analysis, a detailed level of proteomic data.

Limitations

• Small sample size of 51 total tissue specimens may limit statistical power for some analyses.
• The 4.1 MB file size suggests a focused dataset, not a large-scale proteomic repository.
• Data is specific to minor glomerular abnormalities, limiting generalizability to other kidney diseases.

Provenance

Source

Yingying Ling via figshare.

Collection Method

Laboratory-developed pressure cycling technology-based quantitative glycoproteomics workflow integrated with high-resolution mass spectrometry.

Time Range

null

Freshness

Last updated March 2026.

Geography

Human kidney tissues; specific location unknown.