Rabdosin B: Mechanism in Non-Small Cell Lung Cancer

Experimental data and analysis investigate the anti-cancer mechanism of Rabdosin B, a compound from Isodon japonicus, against non-small cell lung cancer (NSCLC). The dataset supports findings that Rabdosin B inhibits NSCLC cell proliferation and migration, induces apoptosis, and synergistically enhances cisplatin sensitivity by targeting the SRC/PI3K/AKT signaling pathway. It includes results from in vitro assays, network pharmacology, molecular docking, molecular dynamics simulations, and in vivo xenograft models.

Use Cases

• Validating drug-target interactions for Rabdosin B based on molecular docking and cellular thermal shift assay (CETSA) data.
• Analyzing synergistic drug effects using combination index calculations from King’s formula.
• Investigating signaling pathway inhibition through Western blot analysis results of SRC/PI3K/AKT proteins.
• Benchmarking in vitro cancer cell responses (proliferation, apoptosis, migration) against a novel compound.

Strengths

• Dataset is openly licensed under CC-BY-4.0, permitting broad reuse.
• Research employs a multi-method validation strategy including network pharmacology, molecular dynamics simulation, and in vivo models.
• Findings are specific, detailing a novel mechanism of action for Rabdosin B via the SRC/PI3K/AKT pathway.

Limitations

• Column names and a precise row count are not provided, limiting understanding of the dataset's internal structure.
• The dataset size (1,292,736 bytes) is given, but its composition across file formats (PDF, DOCX) is not detailed.
• Metadata is sparse, lacking information on the organizing institution and specific temporal coverage of the experiments.

Provenance

Source

Xinfei Chen

Collection Method

Data generated from laboratory experiments including CCK-8, colony formation, flow cytometry, scratch, Transwell, Western blot, network pharmacology, molecular docking, molecular dynamics simulation, cellular thermal shift assay, and xenograft models.

Freshness

Last updated on 2026-03-20.