Col'RFix: Mouse Colon Lipidomics Data from High-Resolution Mass Spectrometry Imaging

A dataset supporting the validation of the Col'RFix tissue preparation protocol for mouse colon. It contains data from mass spectrometry imaging runs at 5, 10, and 20 μm pixel sizes, using multiple MALDI-MSI modalities and ion modes. The data was authored by Sabina H. Skov and last updated in April 2026.

Use Cases

• Compare molecular signal preservation between fixed and unfixed tissue based on spectral comparisons mentioned in the description.
• Analyze spatial localization of lipids at cellular-scale resolution based on the 5 μm pixel size imaging capability.
• Evaluate morphology preservation in colon tissue sections based on correlation with H&E staining results.
• Assess protocol reproducibility across multiple imaging runs and modalities as described in the validation.

Strengths

• Enables high-resolution imaging down to a 5 μm pixel size.
• Protocol validated using replicate sections from two high-fat diet mice and an independent cohort of six mice.
• Data compares fixed and unfixed tissues sampled within 1 cm of the same region.

Limitations

• Row count is unknown, which may limit suitability assessment.
• Column-level documentation is absent; field semantics must be inferred after download.
• The dataset is very small at 21.3 KB, indicating limited scope.

Provenance

Source

figshare

Collection Method

Data generated from mass spectrometry imaging of mouse colon tissue using the described Col'RFix protocol.

Time Range

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Freshness

Last updated 2026-04-13 12:34:45; freshness should be verified.

Geography

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