Col'RFix: Mouse Colon Lipidomics Data from High-Resolution Mass Spectrometry Imaging

A 21.0 KB dataset from figshare, authored by Sabina H. Skov and last updated in April 2026, describes a rapid cold fixation protocol for mouse colon tissue. The data likely contains results from validation experiments assessing morphology preservation, analyte diffusion, and molecular coverage across multiple mass spectrometry imaging runs. Feasibility was evaluated using an independent cohort of six mice, split between lean and high-fat diet groups.

Use Cases

• Benchmarking tissue fixation protocols based on the described assessment of morphology preservation and molecular coverage.
• Analyzing spatial lipidomics patterns in mouse colon based on the mention of imaging down to 5 μm pixel size.
• Comparing molecular profiles between fixed and unfixed tissues based on the spectral comparison described.
• Correlating molecular features with histological details based on the precise correlation enabled by the protocol.

Strengths

• Protocol validation involved multiple MSI runs at 5, 10, and 20 μm pixel sizes, three MALDI-MSI modalities, and both ion modes.
• Biological comparison was evaluated in an independent cohort of six mice (n=3 lean, n=3 high-fat diet).
• The method enabled reproducible imaging down to a 5 μm pixel size, supporting high-resolution spatial analysis.

Limitations

• Column-level documentation is absent; field semantics must be inferred after download.
• Row count is unknown, which may limit suitability assessment.
• The dataset is small (21.0 KB), indicating limited scope, likely containing summary or methodological data rather than raw spectra.

Provenance

Source

figshare

Collection Method

Data generated from mass spectrometry imaging validation of a novel tissue fixation protocol (Col'RFix).

Time Range

Temporal coverage of the underlying experiments is not specified.

Freshness

Last updated 2026-04-13 12:34:44; freshness should be verified.

Geography

Spatial coverage is not specified, but the biological subject is mouse colon tissue.