Validation Data for Rapid Antibiotic Screening Methods in Chicken Feed

Validation data from a study evaluating rapid screening methods for veterinary antibiotics in animal feed. The study validated a microbial inhibition assay and three lateral-flow tests using blank feed samples from Belgian suppliers and 124 commercial broiler feed samples collected in Kenya in November–December 2022. CCβ values were established for twelve antibiotic classes, and LC-MS/MS confirmed specific antibiotics in field samples.

Use Cases

• Compare detection performance of microbiological inhibition assays and lateral flow tests based on CCβ values
• Assess the feasibility of repurposing milk screening methods for feed analysis based on validation results
• Identify antibiotic contamination in commercial feed samples based on LC-MS/MS confirmation data
• Evaluate method stability for practical monitoring based on 24-hour stability assessment

Strengths

• Validation includes 124 commercial broiler feed samples from a specific time period (November–December 2022)
• CCβ values were established for twelve antibiotic classes, providing detection thresholds
• LC-MS/MS confirmation identified tylosin, quinolones, and five sulphonamides in 12 of 124 field samples

Limitations

• Column-level documentation is absent; field semantics must be inferred after download
• Row count is unknown, which may limit suitability assessment
• The dataset is very small (89.7 KB), indicating limited scope

Provenance

Source

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Collection Method

Blank feed samples were spiked, and commercial samples were screened using validated methods.

Time Range

November–December 2022

Freshness

Last updated 2026-05-22 16:59:56; freshness should be verified

Geography

Feed samples from Belgian suppliers and Kenyan commercial sources