Sucrose Permeation into Rice Callus Cells During Cryopreservation

Sucrose permeated rice callus cells within 6 seconds of exposure and reached a steady-state in 20-30 seconds, as tracked by deuterated sucrose and CARS microscopy. This dataset evaluates sucrose permeation for each step of a model cryopreservation cycle. The research was authored by Albert Ochoa-Castillo and uploaded to figshare in April 2026.

Use Cases

• Optimizing plant vitrification solution (PVS) formulations based on measured sucrose permeation kinetics.
• Modeling fundamental cell-sucrose interactions during cryoprotectant exposure.
• Designing future cryopreservation studies on organized plant tissues based on callus cell findings.
• Benchmarking the effectiveness of sucrose as a permeating cryoprotecting agent in plant cells.

Strengths

• Uses a highly sensitive, non-invasive vibrational technique (CARS microscopy) for tracking.
• Provides specific kinetic measurements (6-second initial permeation, 20-30 second steady-state).
• Explicitly evaluates permeation for each step of a model cryopreservation cycle.

Limitations

• Dataset size is listed as 0.0 B, indicating a very limited scope or placeholder entry.
• Row count and column-level documentation are unknown, limiting suitability assessment.
• Description metadata is limited; actual data quality requires manual inspection after download.

Provenance

Source

figshare, authored by Albert Ochoa-Castillo.

Collection Method

Data likely generated from experiments using deuterated sucrose and coherent anti‑Stokes Raman scattering (CARS) microscopy on rice callus cells.

Freshness

Last updated 2026-04-22 06:37:38; freshness should be verified.