Genome Editing Efficiency in Nicotiana benthamiana via Amplicon Sequencing

Two datasets containing raw amplicon sequencing reads from Nicotiana benthamiana leaves where 20 and 32 different gRNAs were co-expressed with SpCas9. The 7.4 GB dataset, authored by Zheng Gong and last updated in April 2026, accompanies a publication with DOI 10.1007/s00299-026-03820-x. It includes barcode files for demultiplexing and metadata for CRISPResso2 analysis.

Use Cases

• Quantifying genome editing efficiency based on amplicon sequencing reads from gRNA targets.
• Analyzing mutation spectra and patterns based on sequencing data from biological replicates.
• Benchmarking gRNA performance based on data from 52 total gRNAs targeting over 10 genes.
• Demultiplexing pooled sequencing runs based on provided barcode sequence files.

Strengths

• Includes data for 52 total gRNAs targeting over 10 genes in Nicotiana benthamiana.
• Contains biological replicates and unedited controls for comparative analysis.
• Provides barcode files and metadata specifically formatted for CRISPResso2 analysis.

Limitations

• Column-level documentation is absent; field semantics must be inferred after download.
• Row count is unknown, which may limit suitability assessment for specific analyses.

Provenance

Source

figshare, accompanying a publication with DOI 10.1007/s00299-026-03820-x.

Collection Method

Amplicon sequencing of PCR-amplified target sites from transiently transformed plant leaves.

Freshness

Last updated 2026-04-25 00:26:42