Neferine-Gentamicin Synergy: Mechanisms Against E. coli

Yasong Yan's research dataset investigates the synergistic effect of the plant alkaloid Neferine with the antibiotic gentamicin against Escherichia coli. The data supports findings that Neferine targets the FNR and ArcA transcriptional regulators, inducing metabolic collapse and membrane disruption to overcome resistance. Results include in vitro and in vivo validation of the combination's efficacy in reducing bacterial load and improving survival.

Use Cases

• Analyzing synergistic drug interactions based on Minimum Inhibitory Concentration (MIC) fold-reduction data.
• Studying bacterial metabolic disruption based on TCA cycle hyper-activation, NAD+/NADH imbalance, and ATP depletion metrics.
• Investigating membrane integrity effects based on fluidity and depolarization measurements.
• Modeling resistance development delay based on in vitro kinetics data.
• Evaluating in vivo therapeutic efficacy based on host survival and organ bacterial load reduction.

Strengths

• Dataset is openly available under a CC-BY-4.0 license, facilitating reuse.
• Research integrates multiple methodological approaches, including high-throughput screening, bioinformatics, and molecular biology.
• Mechanistic insights are supported by specific affinity measurements (K_D = 1.3 μM and 658.6 nM for FNR and ArcA).

Limitations

• Column names and row counts are not provided, limiting understanding of the data's structure and scale.
• The dataset's primary files are in XLSX and ZIP formats, which may require specific software to access.
• The 'last updated' date of 2026-03 25 is anomalously future-dated, casting doubt on temporal metadata accuracy.

Provenance

Source

yasong yan

Collection Method

Integrated high-throughput screening with bioinformatic and molecular biological approaches.

Time Range

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Freshness

2026-03-25 10:56:24

Geography

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