Raw Mass Spectrometry Data for Mouse Plasma Pharmacokinetics of Anisodine

Raw_Data of mass spectrometry for the plasma concentration determination of anisodine contains the raw instrument output files from a pharmacokinetic study in mice. The data was generated by Anqing Li and last updated on 2026-04 23. It consists of 19.4 MB of .wiff and .scan files from an AB SCIEX API 4000 QTRAP mass spectrometer used to measure plasma concentrations of anisodine over a 4-hour period after intravenous injection.

Use Cases

• Method development for liquid chromatography-mass spectrometry (LC-MS) quantification based on the described gradient separation and MRM parameters.
• Pharmacokinetic modeling of drug half-life based on the plasma concentration time points (5 min to 4 hours).
• Reproducibility analysis of bioanalytical assays based on the detailed sample preparation and instrument optimization steps.
• Training data for processing raw mass spectrometry files based on the provided .wiff and .scan formats.

Strengths

• Detailed methodological description including specific instrument model (AB SCIEX API 4000 QTRAP), column specifications, and gradient program.
• Data covers a defined pharmacokinetic time course with six time points over 4 hours from six male C57BL/6J mice.
• Raw data files (19.4 MB) are provided in native instrument formats (.wiff, .scan) suitable for reprocessing.

Limitations

• Column-level documentation is absent; field semantics must be inferred after download.
• Row count is unknown, which may limit suitability assessment.
• Data is from a single, small-scale animal study (n=6), limiting statistical power.

Provenance

Source

figshare, author Anqing Li

Collection Method

Plasma samples from mice administered anisodine were processed and analyzed via liquid chromatography coupled with triple quadrupole mass spectrometry.

Time Range

Experimental time points: 5 minutes, 15 minutes, 30 minutes, -1 hour, 2 hours, and 4 hours post-injection.

Freshness

Last updated 2026-04-23 14:37:55; freshness should be verified.

Geography

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