MbovP0592: Bovine Macrophage TLR Signaling and Cytokine Response Data

Experimental data supporting a study on the inflammatory mechanism of Mycoplasma bovis lipoprotein MbovP0592. The dataset likely contains results from RT-qPCR, Western blotting, immunofluorescence, siRNA knockdown, and co-immunoprecipitation assays performed on BoMac cells. The data was uploaded by Xiaotan Yuan on figshare in May 2026.

Use Cases

• Analyze cytokine expression levels (IL-1β, IL-6, IL-8, TNF-α) based on stimulation time points mentioned in the description
• Investigate TLR2/MyD88/TRAF6/NF-κB pathway activation based on Western blot and functional assay results
• Evaluate the role of TLR10 in negative feedback regulation based on siRNA knockdown and promoter motif prediction experiments

Strengths

• Data is associated with a detailed methodological description combining multiple experimental techniques
• The dataset is 2.4 MB in size, suggesting a focused collection of results

Limitations

• Row count is unknown, which may limit suitability assessment
• Column-level documentation is absent; field semantics must be inferred after download

Provenance

Source

figshare

Collection Method

Experimental data generated from in vitro assays using BoMac cells, including RT-qPCR, Western blotting, immunofluorescence, siRNA knockdown, and co-immunoprecipitation.

Freshness

Last updated 2026-05-20 11:25:02