Raw Flow Cytometry Data for Lipid Droplets in Three Breast Cancer Cell Lines

Raw flow cytometry data (FCS files) of intracellular lipid droplet fluorescence in three breast cancer cell lines: ZR75-1, MCF-7, and MDA-MB-231. The dataset includes fluorescence intensity measurements (FL1 channel) for each cell line and is 16.1 MB in size. It was authored by Yihui Fang and last updated on 2026-04-28.

Use Cases

• Compare lipid droplet content across ZR75-1, MCF-7, and MDA-MB-231 cell lines based on the described fluorescence intensity measurements.
• Train or validate cell population gating algorithms based on the raw FCS file format and FL1 channel data.
• Investigate correlations between lipid droplet fluorescence and other cellular phenotypes in breast cancer models.

Strengths

• Data is provided in the raw FCS file format, which is a standard for flow cytometry and preserves instrument-level detail.
• The dataset includes measurements from three distinct breast cancer cell lines (ZR75-1, MCF-7, MDA-MB-231), allowing for comparative analysis.
• The dataset is licensed under CC-BY-4.0, permitting broad reuse with attribution.

Limitations

• Column-level documentation is absent; field semantics must be inferred after download.
• Row count is unknown, which may limit suitability assessment for large-scale statistical modeling.
• Description metadata is limited; actual data quality requires manual inspection after download.

Provenance

Source

figshare

Collection Method

Cells were stained with BODIPY 493/503 and analyzed on a BD flow cytometer.

Freshness

Last updated 2026-04-28 12:44:08; freshness should be verified.