Raw Flow Cytometry fcs Files for Engineered Bacterial Strains

Raw Flow Cytometry fcs files contain data from experiments on three bacterial species: Corynebacterium glutamicum, Pseudomonas simiae, and Klebsiella michiganensis. The dataset, authored by Brady Cress and last updated on 2026-06-03, includes samples with either an empty vector or a GFP-RFP-kanR construct, each with two replicates. The 19.1 MB collection is shared under a CC-BY-4.0 license on figshare.

Use Cases

• Benchmarking cell population gating algorithms based on fluorescence signals from GFP and RFP markers.
• Training models to classify bacterial species based on flow cytometry profiles.
• Analyzing the effect of genetic constructs (empty vector vs. GFP-RFP-kanR) on cellular properties.
• Developing methods for replicate analysis and technical variability assessment in flow cytometry data.

Strengths

• Includes data for three distinct bacterial species, allowing for cross-species comparison.
• Contains technical replicates (two per condition) for assessing measurement consistency.
• Explicitly documents the genetic pathway (pHGT) condition for each sample file.

Limitations

• Column-level documentation is absent; field semantics must be inferred after download.
• Row count is unknown, which may limit suitability assessment for large-scale ML tasks.
• The 19.1 MB size suggests a relatively small-scale experimental dataset.

Provenance

Source

Brady Cress via figshare.

Collection Method

Likely generated from laboratory flow cytometry experiments on engineered bacterial strains.

Freshness

Last updated 2026-06-03 19:23:27; freshness should be verified.