Medicago truncatula Gene Expression Data Under Temperature and Biotic Stress

Raw quantitative PCR data and calculations for a plant–aphid–AM fungal interaction study. The dataset includes primer efficiency assays and Ct values for target genes MtICS1 and MtAOS1 normalized against EF1α. It was authored by N.M Asif Billah and last updated on 2026-05-17.

Use Cases

• Calculate relative gene expression based on raw Ct values and normalization steps described.
• Validate primer efficiency for qPCR assays in Medicago truncatula based on serial dilution data.
• Analyze the interaction effects of temperature, AM fungal colonization, and aphid infestation on plant defense gene expression.
• Replicate downstream analysis steps for gene expression estimation as outlined in the dataset description.

Strengths

• Experimental design includes three controlled variables: temperature (24°C and 27°C), AM fungal colonization (Mock vs. AMF), and aphid infestation (0 vs. 15 aphids).
• Dataset includes both raw Ct values and the primer efficiency calculations required for accurate expression analysis.
• Gene expression analysis is provided for specific target genes (MtICS1 and MtAOS1) normalized against a reference gene (EF1α).

Limitations

• Column-level documentation is absent; field semantics must be inferred after download.
• Row count is unknown, which may limit suitability assessment.
• The dataset is small (65.6 KB), indicating a limited experimental scope.

Provenance

Source

figshare

Collection Method

Generated from laboratory qPCR experiments.

Freshness

Last updated 2026-05-17 17:08:49; freshness should be verified.