Demultiplexed raw reads from a COI Illumina NextSeq metabarcoding library, including true samples, positive controls, and negative controls. The data was assessed using FastQC and processed with the R package dada2 for Amplicon Sequence Variant generation. Downstream analysis was performed using phyloseq and formatted for ecological analyses with packages like vegan.
Use Cases
- Generate Amplicon Sequence Variants (ASVs) based on the described processing pipeline using the R package dada2.
- Assess moth diversity and community composition based on the COI metabarcoding data mentioned in the description.
- Perform ecological analyses, such as alpha and beta diversity calculations, based on the use of the R packages phyloseq and vegan.
- Evaluate sequencing and laboratory contamination based on the included positive and negative control samples.
Strengths
- Includes positive and negative controls, which can help assess data quality and contamination.
- Processing and analysis steps are documented, mentioning specific tools like FastQC, dada2, phyloseq, and vegan.
- Released under the CC-BY-4.0 license, permitting reuse with attribution.
Limitations
- Description metadata is limited; actual data quality requires manual inspection after download.
- Column-level documentation is absent; field semantics must be inferred after download.
- Row count and file size are unknown, which may limit suitability assessment.
Provenance
- Source
- Alejandro Maeda Obregon via figshare.
- Collection Method
- Illumina NextSeq sequencing of COI metabarcoding amplicons.
- Time Range
- null
- Freshness
- Last updated 2026-04-28 12:45:36; freshness should be verified.
- Geography
- null