Bovine Oocyte and Blastocyst RNA-seq Counts from Millifluidic and Conventional Culture

Raw RNA sequencing count matrices from two experiments on bovine in vitro oocyte maturation. The data includes counts from cumulus-oocyte complexes matured under three conditions and from individual blastocysts derived from two conditions. The dataset was created by Marcia de Almeida Monteiro Melo Ferraz and last updated on March 17, 2026.

Use Cases

• Perform differential expression analysis based on raw RNA-seq counts from oocytes matured under different culture conditions.
• Investigate gene set enrichment related to mechanotransduction pathways in early embryos.
• Compare transcriptional profiles between pooled oocyte samples and individual blastocysts.
• Benchmark analysis pipelines for low-input RNA-seq data from bovine reproductive cells.

Strengths

• Includes five biological replicates per condition for the oocyte experiment, as stated in the description.
• Raw counts were generated by mapping to the bovine reference genome (Ensembl) using STAR and featureCounts.
• Data is provided in a 2.7 MB CSV format, facilitating direct computational analysis.

Limitations

• Row count is unknown, which may limit suitability assessment for some statistical methods.
• Column-level documentation is absent; field semantics must be inferred after download.
• The sample size for the blastocyst experiment is four biological replicates per group, which is a smaller cohort.

Provenance

Source

figshare

Collection Method

RNA sequencing of bovine oocytes and blastocysts from ovaries obtained from a local abattoir, with libraries prepared using specified kits and sequenced on Illumina platforms.

Freshness

Last updated 2026-03-17 20:34:29.