Layer-Specific Neocortical Calcium Imaging in Awake Mice with 26 Subjects

Harvard Dataverse hosts a dataset accompanying a study on layer-specific wide-field calcium imaging in awake mice. It contains processed data and analysis outputs from 26 adult male mice (1–4 months old), including 6 wild-type and 20 triple-transgenic mice expressing GCaMP6f in excitatory neurons of cortical layers L2/3, L5, and L6. The data supports reproduction of findings on light scattering, resting-state functional connectivity, and single-whisker stimulation responses.

Use Cases

• Modeling depth-dependent light scattering in brain tissue based on radial intensity profiles and Monte Carlo simulation code.
• Analyzing layer-specific resting-state functional connectivity patterns based on radial fluorescence trace profiles and connectivity matrices.
• Assessing cortical activation spread from sensory stimuli based on imaging traces with and without spatial deconvolution.
• Comparing region-to-region connectivity and correlation magnitude across cortical layers L2/3, L5, and L6.

Strengths

• Includes data from 26 adult male mice, providing a multi-subject foundation for analysis.
• Contains three distinct, processed components: light scattering characterization, resting-state connectivity, and stimulation responses.
• Uses transgenic mouse lines (JAX #016198, #024103, #022864) for layer-specific targeting of excitatory neurons.
• Provides experimentally-derived spatial deconvolution kernels to improve layer-specific accuracy.

Limitations

• Column-level documentation is absent; field semantics must be inferred after download.
• Row count is unknown, which may limit suitability assessment for large-scale modeling.
• Data may reflect bias inherent to the specific transgenic mouse models and experimental protocols used.

Provenance

Source

Harvard Dataverse, authored by Lorenzo Mercado and Dayra Ayerim.

Collection Method

Processed data from layer-specific wide-field calcium imaging experiments in awake mice.

Time Range

Experimental data collection timeframe is not specified.

Freshness

Last updated 2026-04-09 12:48:46; freshness should be verified.

Geography

Spatial coverage is not specified, but data is from mouse neocortex.