Replication Data for: The interrelation between the 3D microenvironment and mechanics of h

Confocal microscopy images capture single cells and small clusters of induced pluripotent stem cell-derived endothelial progenitor cells (iPSC-EPs) cultured for 4 or 7 days. The dataset includes matched images under basal conditions and after treatment with the actin inhibitor cytochalasin-D, with cells embedded in hydrogels of three different stiffnesses (190 Pa, 336 Pa, 551 Pa). Data was collected by Toni West using a Nikon AXR confocal microscope and is hosted by the Texas Data Repository.

Use Cases

• Quantifying cell-generated traction forces based on hydrogel displacement data from fluorescent bead channels.
• Analyzing morphological changes in iPSC-EPs based on tdTomato-labeled cell images before and after cytoskeletal disruption.
• Correlating hydrogel stiffness (25%, 50%, 75% cross-linking) with cell cluster formation and nuclear count from Hoechst staining.
• Training computer vision models for cell segmentation and tracking using the three-channel (red, blue, green) confocal images.

Strengths

• Images were acquired under controlled conditions with a heated chamber set to 37°C.
• Data includes matched pairs for each condition: basal state and after treatment with cytochalasin-D.
• Three distinct hydrogel stiffness levels (approximately 190 Pa, 336 Pa, and 551 Pa) provide a mechanical gradient for analysis.

Limitations

• Column-level documentation is absent; field semantics must be inferred after download.
• Row count is unknown, which may limit suitability assessment for large-scale machine learning.
• Description metadata is limited; actual data quality and file organization require manual inspection after download.

Provenance

Source

Texas Data Repository Harvested Dataverse

Collection Method

Data was collected on a Nikon AXR confocal microscope equipped with a Queensgate piezo stage and NIS Elements software.

Time Range

Cells were cultured for 4 or 7 days; specific collection dates are not provided.

Freshness

Last updated 2026-04-27 04:13:37; freshness should be verified.

Geography

Spatial coverage is not specified; likely from a laboratory setting.