Staphylococcus Aureus Physiology and Agr Activity Under Low Shear Modelled Microgravity

NASA's dataset investigates the response of Staphylococcus aureus physiology and Agr quorum sensing to low shear modelled microgravity (LSMMG) using the rotary cell culture system (RCCS). The data was generated from cultures grown in BRIC-23 medium and temperature conditions, comparing LSMMG, normal gravity control, and inverted gravity control orientations. Analysis includes metabolite assays for lactate and acetate excretion and secretomics analysis of culture supernatants.

Use Cases

• Modeling bacterial gene expression changes under simulated microgravity based on Agr quorum sensing system data.
• Analyzing metabolic shifts in bacteria under altered oxygen availability based on lactate and acetate excretion measurements.
• Comparing virulence factor regulation across different gravity orientations based on secretomics analysis of culture supernatants.
• Validating ground-based microgravity models for spaceflight microbiology research based on the study's comparison of RCCS orientations.

Strengths

• Data originates from the National Aeronautics and Space Administration (NASA), a leading space research institution.
• Study design includes multiple experimental conditions: LSMMG, normal gravity control, and an inverted gravity control.
• Analysis incorporates multiple omics approaches, including metabolite assays and secretomics.

Limitations

• Column-level documentation is absent; field semantics must be inferred after download.
• Row count is unknown, which may limit suitability assessment.
• File format is listed as BIN, which may require specific tools for access and interpretation.

Provenance

Source

National Aeronautics and Space Administration

Collection Method

Experimental data from cultures grown in the rotary cell culture system (RCCS) under BRIC-23 medium and temperature conditions.

Time Range

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Freshness

Last updated 2026-03-13 20:07:04.464468; freshness should be verified.

Geography

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