RF1 and RF2 Release Factor Conformations Captured by Time-Resolved Cryo-EM

Time-resolved cryo-EM visualizations of transient compact forms of release factors RF1 and RF2 during peptide chain termination on the ribosome. The dataset includes structures at 3.5 and 4 Å resolution, capturing reaction states at 24 ms and 60 ms time points. The data was produced by Ziao Fu from Columbia University and is available via paperswithcode.

Use Cases

• Modeling the conformational change of release factors based on the described compact-to-extended transition.
• Training machine learning models for protein structure prediction using high-resolution cryo-EM maps.
• Analyzing molecular dynamics of stop codon recognition based on the time-resolved reaction pathway.
• Studying the interaction geometry between the GGQ motif and the peptidyl transfer center described in the data.

Strengths

• Includes high-resolution structures at 3.5 Å and 4 Å.
• Captures specific reaction time points at 24 ms and 60 ms.
• Quantifies the population of complexes in the compact state (about 25% at 24 ms).

Limitations

• Column-level documentation is absent; field semantics must be inferred after download.
• Row count is unknown, which may limit suitability assessment.
• Last update date is unknown; freshness unverified.

Provenance

Source

paperswithcode

Collection Method

Time-resolved cryo-electron microscopy.