RNA-based full length immunoglobulin light chain sequencing reveals lambda constant domain

RNA-based full-length light chain repertoire sequencing from 89 patients with systemic light chain amyloidosis, performed with Oxford Nanopore and Illumina technologies. The dataset includes computational analyses of conservation, free energy, and molecular dynamics simulations to investigate thermodynamic stability. It was authored by Eyad Naser and last updated on June 4, 2026.

Use Cases

• Identify lambda constant domain mutations based on the described sequencing results
• Analyze potential thermodynamic stabilizing or destabilizing effects using the described free energy change calculations
• Correlate light chain protein detection in serum and amyloid deposits with sequencing data as described
• Validate full-length light chain sequencing methods for clinical implementation in plasma cell dyscrasias

Strengths

• Sequences showed 100% identity between Oxford Nanopore and Illumina platforms across all patients.
• Monoclonal light chain detection rate was 95.4% in the cohort of 89 patients.
• Lambda constant domain mutations were specifically identified in 10% of cases.

Limitations

• Column-level documentation is absent; field semantics must be inferred after download.
• Row count is unknown, which may limit suitability assessment.
• The 17.0 MB size suggests a relatively small dataset, potentially limiting statistical power.

Provenance

Source

figshare

Collection Method

RNA-based full-length light chain repertoire sequencing with Oxford Nanopore and Illumina, plus mass spectrometry and computational analysis.

Freshness

Last updated 2026-06-04 16:40:05