Julijus Bogomolovas provides gene-level differential expression results from bulk RNA-sequencing of embryonic day 10.5 mouse hearts. The data compares cardiomyocyte-specific Pkn2 conditional knockout embryos to littermate controls, with analysis performed using the DESeq2 pipeline. The dataset includes normalized expression values for biological replicates and statistical metrics.
Use Cases
- Identify genes upregulated or downregulated in PKN2 knockout hearts based on the log2FoldChange and sig columns.
- Validate differential expression findings using the replicate-level normalized expression values (CT1-4, KO1-4).
- Perform downstream bioinformatics analysis, such as pathway enrichment, using the gene-level statistical metrics (pvalue, padj).
Strengths
- Includes normalized expression values for four biological replicates per condition (control and knockout).
- Statistical metrics are provided from a DESeq2 analysis pipeline, including adjusted p-values.
- Dataset is licensed under CC-BY-4.0, allowing for reuse.
Limitations
- Row count is unknown, which may limit suitability assessment.
- Data is limited to embryonic day 10.5 mouse hearts, a specific developmental stage.
- The dataset size is 58.5 KB, indicating a relatively small scope.
Provenance
- Source
- figshare
- Collection Method
- RNA-seq libraries generated from ventricular tissue of embryonic mouse hearts.
- Freshness
- Last updated 2026-05-05 20:56:27; freshness should be verified.