RNA-Seq Quality Metrics for Human Adipose Stem Cells from FastQC

FastQC quality reports for paired-end RNA sequencing reads from human adipose-derived mesenchymal stromal/stem cells. The data, authored by Himari Matsusaka, was last updated on 2026-05-08. It includes visualizations of Phred quality scores across 150 bp read positions for R1 and R2 reads.

Use Cases

• Assess sequencing run quality based on Phred score distributions across read positions.
• Compare read quality between paired-end read files (R1 and R2) for the same sample.
• Identify potential low-quality regions in sequencing data based on the color-coded quality zones (green, yellow, red).

Strengths

• Data is openly licensed under CC-BY-4.0.
• Quality metrics are specifically for 150 bp paired-end reads, a common sequencing configuration.
• The report distinguishes between high-quality (green) and lower-quality (yellow/red) read regions.

Limitations

• The dataset is small at 4.1 MB, suggesting it may contain summary reports rather than raw sequence data.
• Row count and specific column definitions are unknown, limiting detailed assessment.
• The description metadata is limited; actual data quality requires manual inspection after download.

Provenance

Source

figshare

Collection Method

Likely generated by the FastQC tool from RNA sequencing data.

Freshness

Last updated 2026-05-08 06:37:22; freshness should be verified.