RR1 NASA Validation Flight: Mouse Soleus Muscle Omics Data

NASA's GeneLab project provides transcriptomic and epigenomic data from mouse soleus muscle tissue harvested during the Rodent Research-1 spaceflight experiment. The data assesses the viability of RNA and DNA extracted from archived tissue samples stored at -80°C for at least a year after return from the International Space Station. This investigation was conducted to evaluate the feasibility of multi-omics analyses on spaceflight biological specimens.

Use Cases

• Identify spaceflight-induced gene expression changes in mouse soleus muscle based on transcriptomic data.
• Analyze epigenetic modifications in muscle tissue from spaceflight subjects based on epigenomic data.
• Assess sample viability for future multi-omics studies based on the protocols for RNA and DNA extraction from archived tissues.
• Prioritize future rodent research activities on the ISS based on the scientific value demonstrated by this omics analysis.

Strengths

• Data originates from a controlled NASA spaceflight experiment (Rodent Research-1).
• Tissue samples include multiple types (adrenal glands, quadriceps, gastrocnemius, etc.) with soleus muscle being the focus here.
• Samples were preserved using standardized methods (snap-frozen or RNAlater) and stored at -80°C.

Limitations

• Column-level documentation is absent; field semantics must be inferred after download.
• Row count is unknown, which may limit suitability assessment.
• Data is stored in a BIN file format, which may require specialized tools for access and analysis.

Provenance

Source

National Aeronautics and Space Administration (NASA) GeneLab project.

Collection Method

Tissue harvested from mouse subjects on the first Rodent Research spaceflight experiment, preserved, archived, and later analyzed for RNA and DNA.

Time Range

Tissue samples were stored for at least a year after the RR-1 mission return date.

Freshness

Last updated 2026-03-13 19:21:33.969146; freshness should be verified.

Geography

Samples originated from the International Space Station and were processed on Earth.