NASA RR-1 Validation Flight: Mouse Muscle Multi-Omics Data

NASA's Rodent Research-1 (RR1) Validation Flight dataset contains transcriptomic, proteomic, and epigenomic data from mouse tibialis anterior muscle and other tissues. The data was generated by NASA's GeneLab project from tissues harvested during the first RR spaceflight experiment, preserved, and stored for at least a year at -80°C. The investigation assessed the viability of RNA, DNA, and protein from archived samples for multi-omics analysis.

Use Cases

• Analyzing spaceflight-induced changes in gene expression based on transcriptomic data.
• Investigating protein-level adaptations to microgravity based on proteomic data.
• Studying epigenetic modifications in response to space environments based on epigenomic data.
• Assessing tissue preservation and extraction protocols for future space biology experiments.
• Prioritizing future rodent research activities on the ISS based on multi-omics feasibility.

Strengths

• Data originates from a controlled NASA spaceflight experiment (RR-1).
• Includes multiple omics data types (transcriptomic, proteomic, epigenomic) from the same investigation.
• Tissues were sourced from multiple mouse organs including adrenal glands, quadriceps, and kidney.
• Managed and disseminated by NASA's GeneLab project with a focus on unrestricted data sharing.

Limitations

• Row count and dataset scale are unknown, which may limit suitability assessment.
• Column-level documentation is absent; field semantics must be inferred after download.
• Data is stored in BIN format, which may require specialized tools for access and analysis.

Provenance

Source

National Aeronautics and Space Administration (NASA), GeneLab project

Collection Method

Tissues harvested from mouse subjects during the first Rodent Research spaceflight experiment, snap-frozen or RNAlater-preserved, and stored at -80°C.

Time Range

From the first Rodent Research (RR-1) spaceflight experiment; tissues stored for at least a year post-flight.

Freshness

Last updated 2026-03 13 19:20:48.397796; freshness should be verified.

Geography

International Space Station (ISS) and Earth-based analysis.