RaDR-GFP Mouse Model: Tissue and Age Dependence of Homologous Recombination

The RaDR-GFP mouse model from MIT enables in vivo detection of homologous recombination events across mammalian tissues. Data includes comparisons among 11 tissues, showing recombination frequency varies by over two orders of magnitude, with the brain having the lowest rate. The model also tracks how recombination events accumulate with age in the colon and measures the impact of alkylating agent exposure.

Use Cases

• Quantifying tissue-specific homologous recombination frequency based on flow cytometry data from 11 tissues.
• Analyzing the accumulation of de novo recombination events with age based on longitudinal data from the colon.
• Studying DNA damage-induced homologous recombination susceptibility based on exposure data from the colon, liver, and pancreas.
• Identifying cell types undergoing homologous recombination based on histological analysis data of pancreatic acinar cells, liver hepatocytes, and colonic somatic stem cells.

Strengths

• Enables in vivo detection of homologous recombination, a capability described as previously limited.
• Compares recombination frequency across 11 distinct mammalian tissues.
• Quantifies recombination events using both in-house image analysis software and flow cytometry.

Limitations

• Column-level documentation is absent; field semantics must be inferred after download.
• Row count is unknown, which may limit suitability assessment.
• Last update date is unknown; freshness unverified.

Provenance

Source

Massachusetts Institute of Technology

Collection Method

Data generated from a genetically engineered mouse model (RaDR-GFP) with analysis via in-house image software and flow cytometry.