Federico La Spada designed sequences for a recombinase polymerase amplification assay targeting a 134 bp region of the tef1 locus in Phyllosticta citricarpa. These molecular components support a rapid fluorescence-based workflow for pathogen detection. The dataset is associated with a manuscript submitted to Microbiological Research.
Use Cases
- Validate a fluorescent RPA assay based on the provided primer and probe sequences.
- Assess assay specificity and sensitivity for Phyllosticta citricarpa detection.
- Reproduce the detection workflow described in the associated manuscript.
- Design comparative assays using the 134 bp tef1 locus target region.
Strengths
- Sequences target a specific 134 bp region of the tef1 locus.
- Designed to support assay specificity, sensitivity, and reproducibility.
- Published under a CC-BY-4.0 license for transparency and reuse.
Limitations
- Column-level documentation is absent; field semantics must be inferred after download.
- Row count is unknown, which may limit suitability assessment.
- Data may reflect methodological bias inherent to the specific assay design.
Provenance
- Source
- figshare
- Collection Method
- Sequences were designed for a specific RPA assay.