RR-1 and RR-3: Mouse Liver Transcriptomics with ERCC Spike-in Controls

NASA sequenced total RNA from mouse livers in the Rodent Research 1 and Rodent Research 3 spaceflight missions. The data includes samples from flight, ground control, basal control, and vivarium control groups, with half of the samples spiked with ERCC Mix 1 or Mix 2 control RNA. This dataset is intended to test the utility of ERCC RNA controls for interpreting assay sensitivity and variability in RNA sequencing.

Use Cases

• Benchmarking RNA sequencing assay sensitivity based on the inclusion of known ERCC control transcripts.
• Analyzing gene expression variability between spaceflight and control mouse groups.
• Developing normalization methods for transcriptomics data using ERCC spike-in controls.
• Comparing transcriptomic profiles from different rodent research missions (RR-1 and RR-3).

Strengths

• Includes a standard set of 92 ERCC RNA control transcripts added at known ratios.
• Compares multiple experimental groups: flight, ground control, basal control, and vivarium control.
• Data is associated with specific NASA missions (Rodent Research 1 and 3).

Limitations

• Column-level documentation is absent; field semantics must be inferred after download.
• Row count is unknown, which may limit suitability assessment.
• Data files are in a BIN format, which may require specialized tools for access.

Provenance

Source

National Aeronautics and Space Administration

Collection Method

RNA sequencing of total RNA extracted from mouse livers, with and without added ERCC control RNA.

Freshness

Last updated 2026-03-13 19:39:29.615611; freshness should be verified.