LINE-1 and Alu Methylation Primer Data for Cancer Studies

761.0 KB ZIP file contains primer sets and qPCR conditions for quantifying DNA methylation of LINE-1 and Alu repetitive elements. The data includes correlation analyses between methylation status and clinicopathological characteristics for breast and lung cancer patients. Author Tung The Pham published this supporting material under a CC BY 4.0 license in 2026.

Use Cases

• Design methylation-dependent-specific PCR (MSP) and methylation-independent PCR (MIP) assays using the provided primer sequences for LINE-1 and Alu targets.
• Analyze correlations between LINE-1 or Alu methylation levels in cfDNA and patient age, as referenced in the correlation figures.
• Investigate the relationship between clinicopathological characteristics and LINE-1/Alu methylation status in breast cancer and lung cancer tissues.
• Assess the effect of DNA input amount for bisulfite conversion on PCR amplification efficiency and measured methylation levels for LINE-1 and Alu.

Strengths

• Primer design details are specified, including the replacement of non-CpG cytosines with 't' or 'a' in forward and reverse primers.
• Statistical analysis methods (Unpaired t-test, Spearman’s rank correlation) and significance levels (P-values) are documented for the experimental results.
• The dataset is associated with a recent publication, last updated in March 2026.

Limitations

• The dataset is small in scale at 761.0 KB, indicating limited raw data volume.
• Specific row counts, column names, and sample data are unavailable, restricting direct analytical use.
• The data appears focused on methodological validation and specific correlation tests, not a broad patient cohort.

Provenance

Source

Figshare, authored by Tung The Pham.

Collection Method

Primers designed based on consensus sequences of LINE-1 and Alu; data generated from quantitative real-time PCR and correlation analyses with clinical characteristics.

Freshness

Last updated on 2026-03-23.