S3 Table: Drosophila Protein Evolution Fitness Landscape Data

Flora Borne published an Excel dataset on figshare in June 2026. The data likely contains results from a population genetic screen for spatially-clustered adaptive amino acid substitutions in Drosophila proteins. It includes experimental reconstructions of evolutionary intermediates for the Trio protein to study intramolecular epistasis.

Use Cases

• Analyze patterns of evolutionary rate variation among protein sites based on intramolecular epistasis.
• Identify candidates for functional analysis of spatially-clustered amino acid substitutions.
• Study fitness landscapes and constraints on adaptive protein evolution using reconstructed evolutionary intermediates.
• Investigate the role of recessive deleterious effects in circumventing evolutionary constraints.

Strengths

• Dataset is openly available under a CC-BY-4.0 license.
• Focuses on a specific proof-of-concept protein (Trio) with three spatially-clustered substitutions.
• Data is derived from systematic reconstruction of evolutionary intermediates using genome editing.

Limitations

• Row count is unknown, which may limit suitability assessment.
• Column-level documentation is absent; field semantics must be inferred after download.
• The dataset is very small (5.4 KB), indicating limited scope.

Provenance

Source

figshare

Collection Method

Likely derived from a population genetic screen and systematic genome editing experiments.

Freshness

Last updated 2026-06-03 17:45:53; freshness should be verified.