Sclerotia Microbiome: 65 Samples from Flooding Treatments Over 28 Days

65 PacBio full-length 16S rRNA sequencing samples from sclerotia of Sclerotinia sclerotiorum under two flooding treatments across 7 timepoints. The dataset also includes the genome sequence and annotation of the newly characterized bacterium Trinickia sclerotiorum sp. nov. type strain MC. It was authored by Zhong-Han Shie and last updated on 2026-07-15.

Use Cases

• Contrasting microbial effects on sclerotia mortality based on soil treatment (flooded vs. autoclaved soil).
• Analyzing temporal microbiome succession based on samples collected at 7 timepoints over 28 days.
• Characterizing the novel bacterium Trinickia sclerotiorum based on its provided genome sequence and annotation.
• Studying bacterial community resilience in agricultural soil conditions using 16S rRNA sequencing data.

Strengths

• Includes 65 samples with 5 biological replicates per factorial combination of treatment and timepoint.
• Covers 7 distinct timepoints (1-, 3-, 5-, 7-, 14-, 21-, and 28-day) for longitudinal analysis.
• Provides both microbiome sequencing data and a complete bacterial genome sequence.

Limitations

• Column-level documentation is absent; field semantics must be inferred after download.
• Row count is unknown, which may limit suitability assessment.
• Five samples failed DNA quality checks and are not included in the 65-sample set.

Provenance

Source

Harvard Dataverse, author Zhong-Han Shie.

Collection Method

PacBio full-length 16S rRNA sequencing of sclerotia samples and genome sequencing of a bacterial isolate.

Time Range

Experimental timepoints span 1 to 28 days.

Freshness

Last updated 2026-07-15 06:29:18; freshness should be verified.