SCRIPTS AND ESSENTIALS: Lipid Droplet Analysis Tools for Yeast Microscopy

A collection of scripts and essential files for analyzing lipid droplet size in yeast cells. The package includes LD Toolbox, LD Segmenter, and LD ClusterAnalyser scripts, along with PSF files, an installation guide, and a demo movie. It was authored by Heimo Wolinski and uploaded to figshare in June 2026.

Use Cases

• Segmenting lipid droplets in microscopy images based on the LD Segmenter v1 script.
• Profiling lipid droplet size distributions based on the description of size profiling.
• Analyzing spatial clustering of lipid droplets based on the LD ClusterAnalyser v1 script.
• Applying a pre-trained classifier for image analysis based on the included Ilastik Classifier file.

Strengths

• Includes measured 3D PSF and MIP PSF files for accurate image analysis.
• Provides an installation guide and demo movie for user support.
• Released under a permissive CC-BY-4.0 license.

Limitations

• Description metadata is limited; actual data quality requires manual inspection after download.
• Row count is unknown, which may limit suitability assessment.
• Column-level documentation is absent; field semantics must be inferred after download.

Provenance

Source

Heimo Wolinski, associated with IMB-Graz, University of Graz, and Field of Excellence Graz.

Collection Method

Likely generated from microscopy image analysis of yeast cells.

Freshness

Last updated 2026-06-02 12:12:10; freshness should be verified.