Shigella flexneri Phage-Resistant Mutant Phenotypes and Genomes

Research from Yale University characterizes five phage-resistant mutants of the bacterial pathogen Shigella flexneri. The dataset likely contains phenotypic measurements and genomic sequencing data related to changes in membrane permeability, lipopolysaccharide levels, antibiotic sensitivity, and phage susceptibility. This work investigates the trade-off between phage resistance and virulence attenuation.

Use Cases

• Modeling evolutionary trade-offs between phage resistance and virulence based on phenotypic trait measurements.
• Analyzing genotype-phenotype relationships in bacterial pathogens based on whole genome sequencing of mutants.
• Training models to predict virulence attenuation from mutations in ompA or LPS-core biosynthesis genes.

Strengths

• Data is derived from a controlled experimental study characterizing five distinct phage-resistant mutants.
• Phenotypic analysis includes multiple specific traits: cell-membrane permeability, total LPS, sensitivity to four antibiotics, and susceptibility to two other phages.
• Genotypic data is confirmed by whole genome sequencing linking mutations to specific genes (ompA, gmhA, gmhC).

Limitations

• Row count is unknown, which may limit suitability assessment.
• Column-level documentation is absent; field semantics must be inferred after download.
• Data may reflect experimental bias inherent to the specific laboratory conditions and bacterial strain used.

Provenance

Source

Yale University

Collection Method

Experimental laboratory study involving phenotypic assays and whole genome sequencing of bacterial mutants.