SERINC3 Osteogenesis Study: Molecular and Cellular Data

Zhichao Zheng published experimental data on figshare in 2026 investigating the role of SERINC3 in bone formation. The 10.7 MB dataset includes PDF and XLSX files containing results from RNA sequencing, Seahorse energy metabolism analysis, and assays on bone marrow-derived stem cells and knockout mice. The study identifies SERINC3 as a regulator of osteogenesis via IL-32/AMPK-mediated autophagy and mitochondrial metabolism.

Use Cases

• Analyzing gene expression changes during osteogenic differentiation based on RNA sequencing results mentioned in the description
• Investigating the relationship between mitochondrial energy metabolism and bone formation based on Seahorse analysis and tricarboxylic acid cycle enzyme data
• Modeling the impact of SERINC3 knockout on bone volume and mineral density based on microCT data from calvarial defect models
• Studying the IL32-AMPK-autophagy signaling axis in mesenchymal stem cells based on pathway analysis results

Strengths

• Dataset includes specific quantitative results, such as a 42% reduction in bone volume and a 35% lower bone mineral density in knockout mice
• Experimental data covers multiple methodologies including RNA sequencing, Western blotting, Co-IP, and in vivo models
• Data is published under a permissive CC-BY-4.0 license

Limitations

• Column-level documentation is absent; field semantics must be inferred after download
• Row count is unknown, which may limit suitability assessment
• Data may reflect the specific experimental bias inherent to the figshare platform and the author's research focus

Provenance

Source

Zhichao Zheng

Collection Method

Experimental data generated via lentivirus infection, RT-qPCR, staining, microCT, RNA sequencing, Co-IP, Western blotting, and Seahorse analysis.

Freshness

Last updated 2026-05-15 07:56:33; freshness should be verified