Shotgun Lipidomics of SARS-CoV-2 ORF3a FLP in Hela Cells

Shotgun lipidomics data measures lipid levels in Hela cells expressing SARS-CoV-2 ORF3a proteins. The dataset includes measurements for Total BMP, PG, CL, and lysosomal PG. Baley Goodson contributed this dataset to the Harvard Dataverse, with a last update recorded on 2026-05-14.

Use Cases

• Compare lipid composition based on expression of wild-type SARS-CoV-2 ORF3a versus its W193A mutant.
• Analyze changes in specific lipid classes like BMP, PG, and CL mentioned in the description.
• Investigate the role of SARS-CoV-2 ORF3a in modulating lysosomal phospholipids.
• Validate lipidomic findings related to viral protein function in a controlled cell culture model.

Strengths

• Focuses on specific lipid classes (BMP, PG, CL, lysosomal PG) relevant to viral-host interactions.
• Includes a comparative experimental design with wild-type and mutant (W193A) SARS-CoV-2 ORF3a proteins.
• Hosted on the Harvard Dataverse platform, suggesting institutional oversight.

Limitations

• Column-level documentation is absent; field semantics must be inferred after download.
• Row count is unknown, which may limit suitability assessment for large-scale analyses.
• Description metadata is limited; actual data quality and completeness require manual inspection.

Provenance

Source

Harvard Dataverse

Collection Method

Shotgun Lipidomics performed on Hela cell cultures.

Freshness

Last updated 2026-05-14 01:33:17; freshness should be verified.