Single-Cell Raman Spectra of Piscinibacter sakaiensis During PET Degradation

811 single-cell Raman spectra from the bacterium Piscinibacter sakaiensis, acquired across two experimental cohorts. The data was collected by Jiabao Xu and published on figshare in June 2026. Cohort 1 captures cells before and after 4 weeks of incubation on PET films, while Cohort 2 tests the effects of amino-acid supplementation across biofilm and planktonic phenotypes.

Use Cases

• Detect population-level transcriptomic responses at single-cell resolution based on Raman spectral changes before and after PET incubation.
• Investigate membrane and protein composition changes accompanying PET degradation based on spectral features.
• Test whether targeted amino-acid supplementation drives predicted biochemical changes based on comparisons of control, isoleucine, and serine treatments.
• Identify distinct membrane signatures of biofilm versus planktonic phenotypes based on spectral differences between cohorts.

Strengths

• Contains 811 individual single-cell measurements.
• Data is structured across two distinct experimental cohorts addressing specific biological questions.
• Published under a permissive CC-BY-4.0 license.

Limitations

• Column-level documentation is absent; field semantics must be inferred after download.
• Row count per cohort is unknown, which may limit suitability assessment.
• Data may reflect experimental bias inherent to the specific laboratory setup.

Provenance

Source

figshare

Collection Method

Single-cell Raman spectroscopy of Piscinibacter sakaiensis 201-F6.

Time Range

Experimental data from cohorts conducted in December 2025 and April 2026.

Freshness

Last updated 2026-06-02 14:05:26.