APEX-04-EpEx: Single-Molecule DNA Methylation in Arabidopsis Roots During Spaceflight

NASA's Advanced Plant Experiment-04 - Epigenetic Expression (APEX-04-EpEx) experiment on the International Space Station examined cytosine methylation in Arabidopsis thaliana root tissues. The dataset contains single-molecule methylation profiling from wild-type and elp2-5 mutant plants, generated using Flap-Enabled Next-Generation Capture (FENGC) to target specific genomic regions. Data was last updated on March 13, 2026.

Use Cases

• Compare spaceflight-altered DNA methylation between wild-type and mutant Arabidopsis lines based on the genetic lines described.
• Analyze single-molecule resolution methylation patterns over large gene regions as mentioned in the description.
• Investigate the role of the Elongator Complex Subunit 2 (ELP2) epigenetic regulator in stress response based on the elp2-5 mutant data.
• Develop or benchmark targeted methylation sequencing methods for plant genomes, referencing the described FENGC technique.

Strengths

• Data originates from a controlled NASA experiment on the International Space Station, providing a unique environmental context.
• Profiling was performed at single-molecule resolution for user-defined genomic targets, suggesting high precision for specific regions.
• Includes comparative data from two distinct genetic lines (wild-type Col-0 and elp2-5 mutant).

Limitations

• Column-level documentation is absent; field semantics must be inferred after download.
• Row count and dataset size are unknown, which may limit suitability assessment.
• Data is stored in a BIN format, which may require specialized tools for access and analysis.

Provenance

Source

National Aeronautics and Space Administration

Collection Method

Flap-Enabled Next-Generation Capture (FENGC) sequencing on Arabidopsis root tissues from the APEX-04-EpEx spaceflight experiment.

Time Range

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Freshness

Last updated 2026-03 13 20:06:16.008914; freshness should be verified.

Geography

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