Lysozyme and MTH1-8DG MicroED Data from Cryo-EM Experiments

Three MicroED datasets containing 106 crystals total: 61 for Lysozyme, 20 for MTH1-8DG collected with high fluence, and 25 for MTH1-8DG collected with low fluence. Data was collected using a Titan Krios G2/G3i microscope with a CetaD CMOS detector at 77 Kelvin, processed via AutoLEI with XDS. The dataset is hosted on the eu_open_data platform under a CC-BY-4.0 license and was last updated on April 11, 2025.

Use Cases

• Develop low-dose electron diffraction data processing algorithms based on the MTH1-8DG low-fluence dataset.
• Benchmark protein structure refinement tools using the high-resolution Lysozyme MicroED data.
• Train machine learning models for crystal orientation detection using the continuous rotation frame series.
• Compare radiation damage effects in protein-ligand complexes using the high-dose and low-dose MTH1-8DG datasets.

Strengths

• Includes data from 106 crystals across three distinct experimental conditions.
• Specifies detailed instrumental parameters, including accelerating voltage (300 kV), wavelength (0.01968 Å), and pixel size (5.6 µm).
• Provides explicit experimental conditions for each dataset, such as tilt range, exposure time, and frames per data collection.

Limitations

• Column-level documentation is absent; field semantics must be inferred after download.
• Row count is unknown, which may limit suitability assessment.
• Description metadata is limited; actual data quality requires manual inspection after download.

Provenance

Source

CERN - European Organization for Nuclear Research and European Commission

Collection Method

Data collected with a CMOS CetaD detector on a Titan Krios G2/G3i microscope using EPUD software.

Freshness

Last updated 2025-04-11 00:00:00; freshness should be verified.

Geography

Scilifelab, Stockholm