Confocal Microscopy Images of Tumor Spheroids in Collagen ECM

Snapshot imaging captures tumor spheroids embedded in a 3D type I collagen extracellular matrix with photocrosslinked patches. Data was acquired on a Leica TCS SPE confocal microscope using a 10x air, 0.40 NA objective at day 0 and day 1 post-embedding. The dataset is a 97.1 MB ZIP file containing microscopy images, with specific row and column counts unknown.

Use Cases

• Segment tumor spheroids from the 3D collagen ECM background in confocal microscopy images.
• Analyze morphological changes in spheroids by comparing images from the day 0 and day 1 timepoints.
• Train a model to identify the presence and structure of photocrosslinked ECM patches within the image data.

Strengths

• Imaging performed on a specified Leica TCS SPE confocal microscope with a 10x air, 0.40 NA objective, ensuring consistent acquisition.
• Contains temporal data with snapshots taken at two distinct time points (day 0 and day 1).
• Dataset focuses on a specific experimental model: tumor spheroids in a 3D type I collagen ECM with photocrosslinked patches.

Limitations

• The 97.1 MB size indicates a relatively small image collection, limiting the scale of deep learning applications.
• Lack of provided column names, sample data, or row counts makes initial data exploration and structuring uncertain.
• Limited to two early timepoints (day 0 and day 1), restricting analysis of long-term spheroid dynamics.

Provenance

Source

figshare, author Bo Sun.

Collection Method

Snapshot imaging via confocal microscopy of prepared tumor spheroid samples.

Time Range

Images captured at day 0 and day 1 relative to embedding.

Freshness

Last updated March 13, 2026.

Geography

null