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Environmental DNA metabarcoding data from sediment and water samples collected at 11 locations along the Jequitinhonha River catchment in Brazil. The dataset includes 252 Molecular Operational Taxonomic Units (MOTUs) corresponding to 34 fish species, sampled twice over a five-week period around a major rain event. It was created by Naiara Guimarães Sales of the University of Salford to evaluate eDNA for biomonitoring.
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