PEDV Adaptation Data: Spike Protein Mutations and Replication Kinetics

Wanyu Dong's dataset, last updated May 20, 2026, contains genomic and phenotypic data related to the adaptation of Porcine Epidemic Diarrhea Virus (PEDV) in Vero cells. The data likely includes mutation analysis, plaque morphology measurements, and viral titer comparisons between low-passage and high-growth variants. It supports findings on a key mutation at residue 1378 in the S2 subunit that enhances viral replication.

Use Cases

• Mapping adaptive mutations in the PEDV spike gene based on genomic analysis.
• Comparing viral replication kinetics and plaque morphology between virus variants.
• Evaluating the functional impact of specific S2 subunit mutations on viral fitness in Vero cells.
• Designing reverse genetics experiments for vaccine candidate generation.

Strengths

• Dataset includes data from a reverse genetics system confirming the S protein's role in adaptation.
• Analysis maps a specific adaptive mutation to residue 1378, providing a concrete genetic locus.
• Data supports the successful rescue of a clinical isolate via S2 subunit transfer, demonstrating functional significance.

Limitations

• Column-level documentation is absent; field semantics must be inferred after download.
• Row count is unknown, which may limit suitability assessment.
• The dataset is small at 214.3 KB, indicating limited scope.

Provenance

Source

Wanyu Dong via figshare

Collection Method

Likely generated through serial virus propagation in Vero cells, genomic sequencing, and phenotypic assays.

Freshness

Last updated 2026-05-20 09:04:43; freshness should be verified.