Phase Separation of LacY Membrane Protein in E. coli Microscopy Data

Microscopy files and plate reader data supporting research on liquid-liquid phase separation of the lactose permease (LacY) membrane protein in Escherichia coli. It includes optical and electron microscopy files in .czi, .lsm, .lif, and .tif formats, raw .stk files from SMdM and PALM acquisitions, and .csv output files from a TECAN plate reader. The data is organized by the figures in the associated publication.

Use Cases

• Analyze LacY-PopTag condensate localization patterns from PALM and SMdM .stk files to study protein dynamics.
• Correlate LacY transport activity from TECAN plate reader .csv files with microscopy-based localization data under osmotic stress.
• Process optical and electron microscopy files (.czi, .lsm, .lif, .tif) to visualize membrane-bound biomolecular condensate formation.
• Investigate co-condensation effects on activity by integrating plate reader .csv data with microscopy data for LacY and β-galactosidase LacZ.

Strengths

• Includes multiple microscopy modalities: Photoactivated Localization Microscopy (PALM) and Single Molecule Displacement Mapping (SMdM).
• Data is directly linked to a published study on membrane protein phase separation, providing experimental context.
• Files are organized based on the figures in the publication, facilitating direct validation and reproduction of results.

Limitations

• The dataset size, row count, and specific file sizes are not provided, making it difficult to assess the volume of data.
• Molecular dynamics simulation data and analysis code are stored in a separate Zenodo repository (10.5281/zenodo.17335657), requiring users to access multiple sources.
• Data is focused on a single engineered protein system (LacY-PopTag) in E. coli, limiting generalizability to other membrane proteins or organisms.

Provenance

Source

DataverseNL Harvested Dataverse.

Collection Method

Experimental data from optical/electron microscopy, SMdM/PALM acquisitions, and plate reader assays, as described in the associated publication.

Freshness

Last updated on 2026-02-02.