Structural Diversity and Analytical Characterization of Acylhomocarnitines

Acylhomocarnitines are a previously uncharacterized family of mammalian metabolites, homologs of acyl-carnitines extended by one carbon. Jaclyn Weinberg provides liquid chromatography–mass spectrometry methods and sample preparation protocols for their analysis. The dataset includes enzyme-generated standards and diagnostic product ions to distinguish these compounds from isomeric acyl-carnitines.

Use Cases

• Developing LC-MS methods for metabolite separation based on the described sample preparation and chromatographic methods.
• Identifying and distinguishing acylhomocarnitines from acyl-carnitines based on diagnostic product ions from ion dissociation analyses.
• Studying carnitine analog metabolism in mammalian cells using the described in vitro isotope tracer study framework.
• Analyzing metabolite extracts from mouse tissues using the provided analytical strategies.

Strengths

• Includes specific analytical methods for LC-MS measurement of a previously uncharacterized metabolite family.
• Provides diagnostic product ions to differentiate isomers, a concrete analytical contribution.
• Dataset is openly available under the CC-BY-NC-4.0 license.

Limitations

• Row count is unknown, which may limit suitability assessment.
• Column-level documentation is absent; field semantics must be inferred after download.
• The dataset is very small at 10.8 KB, indicating limited scope.

Provenance

Source

figshare

Collection Method

Liquid chromatography–mass spectrometry analysis of enzyme-generated standards and mammalian cell/tissue extracts.

Freshness

Last updated 2026-05-13 20:05:42; freshness should be verified.