HECTD3 Ligase Structures from X-ray Crystallography and Cryo-EM

Structural biology data contains atomic coordinates and electron density maps for the HECT ligase HECTD3. The dataset includes one X-ray structure of the DOC domain and three full-length cryo-EM structures in apo and ubiquitin-loaded states. Author Jessica Huber published these structures and associated maps in 2026.

Use Cases

• Analyze the DOC domain atomic coordinates from the X-ray structure to understand local protein folding.
• Compare the L- and inverted T-conformations from the apo cryo-EM structures to study conformational dynamics.
• Examine the ubiquitin-loaded HECTD3 cryo-EM structure to model the ubiquitination mechanism.
• Use the sharpened and local filtered cryo-EM maps for model validation and refinement.

Strengths

• Includes multiple experimental structures: one X-ray and three distinct cryo-EM reconstructions.
• Provides associated experimental maps (sharpened and local filtered) for model validation.
• Released under a permissive CC BY 4.0 license for reuse.

Limitations

• Contains no tabular or quantitative biological data, limiting use to structural analysis.
• The 598.8 MB file size indicates a medium-scale dataset focused on a single protein system.

Provenance

Source

figshare, author Jessica Huber.

Collection Method

Experimental structural biology via X-ray crystallography and cryo-electron microscopy.

Time Range

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Freshness

Last updated March 2026.

Geography

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