STS-135: Mouse Liver RNA-Seq Data from Spaceflight

Female C57BL/6CR mice were flown aboard the Space Shuttle STS-135 for a 13-day mission. NASA collected and snap-froze liver tissue samples within hours of landing for GeneLab to perform RNA-Seq analysis with ERCC spike-in controls. The data was last updated on March 13, 2026.

Use Cases

• Identify differentially expressed genes in mouse liver based on spaceflight exposure described in the study.
• Analyze transcriptomic stress responses based on the 13-day mission duration and post-landing sample collection.
• Benchmark RNA-Seq analysis pipelines using the ERCC control spike-ins mentioned in the protocol.

Strengths

• Data originates from a controlled NASA spaceflight mission (STS-135).
• RNA-Seq analysis included ERCC spike-in controls for quality assessment.
• Tissue collection was performed within a precise 3-4 hour window post-landing.

Limitations

• Column-level documentation is absent; field semantics must be inferred after download.
• Row count is unknown, which may limit suitability assessment.
• The dataset is provided in a BIN file format, which may require specialized tools for access.

Provenance

Source

National Aeronautics and Space Administration

Collection Method

RNA extracted from liver tissue of spaceflight-exposed mice and sequenced.

Freshness

Last updated 2026-03-13 19:29:30.361444; freshness should be verified