Decontaminated Amplicon Sequence Tables For Insect Microbiome Analysis

Diana Rojas Guerrero published decontaminated amplicon sequence variant (ASV) and operational taxonomic unit (OTU) tables on April 3, 2026. The 10.0 MB dataset contains processed COI, 16S rRNA, and ITS2 marker gene data from insect microbiome studies. It includes relative abundance calculations and spike-in corrected tables.

Use Cases

• Analyze insect-associated microbial communities using decontaminated ASV and OTU abundance tables.
• Compare fungal (ITS2) to animal (COI) biomass ratios using the provided ITS2/COI ratio estimations.
• Benchmark decontamination methods with the included spike-in corrected 16S rRNA tables at OTU and order taxonomic levels.
• Model relative abundance patterns across different taxonomic groups from the provided tables.

Strengths

• Data is decontaminated, reducing false positives from contamination.
• Includes three marker genes (COI, 16S rRNA, ITS2) for multi-kingdom analysis.
• Provides spike-in corrected abundance tables for quantitative assessment.
• Published under a permissive CC-BY-4.0 license in April 2026.

Limitations

• The 10.0 MB file size indicates a small-scale study with limited sample depth.
• Unknown row count and specific sample origins limit reproducibility assessments.
• Data is provided only in XLSX format, which is not ideal for large-scale programmatic analysis.

Provenance

Source

figshare, authored by Diana Rojas Guerrero.

Collection Method

Generated from amplicon sequencing data (COI, 16S rRNA, ITS2) followed by bioinformatic decontamination and processing.

Time Range

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Freshness

Last updated April 3, 2026.

Geography

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